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TM

MERCURIUS   BRB-seq service
 

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MERCURIUS   BRB-seq service
From RNA extraction to data analysis.

TM

Our service extends the cost and throughput benefits of BRB-seq to any user, also those that do not currently possess the required facilities or expertise.

Depending on the user's needs, we will run the service directly we will or help implement BRB-seq at their service provider/core facility of choise. 

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As opposed to standard RNA-seq - where all samples need to be processed individually throughout the library preparation workflow - with BRB-seq all samples can be pooled and processed in one single tube right after the first reverse transcription reaction.

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TM

Our optimized BRB-seq    barcodes facilitate robust and uniform NGS data distribution across large numbers of samples.

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TM

Each kit contains unique BRB-seq    barcodes that are designed to "tag" in the a sample-specific manner all the mRNA molecules of a given sample.

Once all the samples have been barcoded, they can be immediately pooled together in one tube, which drastically reduces costs and manual operations of the downstream steps and, as a consequence, of the overall workflow.

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Once all the samples have been barcoded, they can be immediately pooled together in one tube, which drastically reduces costs and manual operations of the downstream steps and, as a consequence, of the overall workflow.

TM

They chose BRB-seq

"BRB-seq was a great way for us to multiplex large numbers of RNA samples for sequencing. We wanted a gene expression curve with a tight temporal resolution, thus, many time points for several conditions. We are very happy with the quality of the results and with the cost benefit.”

"BRB-seq is very cost-effective and it enables accurate comparison of RNA levels among a large number of samples."

"BRB-seq allowed us to plan an experiment with ten times more replicates than usual, and gain insight into the variability and control of gene expression in fly embryogenesis"

"BRB-seq provided us with a cost-effective way to quantify transcriptomic changes from a large set of samples."

Dr. Nuno Miguel Luis, CNRS Researcher

Dr. Hirokazu Okada, ETH Zurich

Prof. Dr. Marc Robinson-Rechavi, University of Lausanne

Prof. Dr. Martin Klingenspor, TUM Munich

Ready to scale-up your RNA-seq project?