BRB-seq kit

24, 96, 384 RNA-seq library preps in one tube.

The MERCURIUS™ BRB-seq kits contain all the oligos and enzymes needed to go from purified RNA to sequencing-ready DNA libraries.

The generated 3' mRNA-seq data is ideally suited for differential gene expression analysis.


As compared to other mRNA-seq solutions, BRB-seq makes it possible to process virtually any number of RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs. 

Each kit contains enough reagents (including Unique Dual Indexing oligos) to prepare up-to three sequencing libraries.

After sequencing, the generated data can be easily demultiplexed and aligned to the genome of choice using our dedicated cloud-based pipeline, which does not require prior bioinformatic experience.

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Bulk RNA sequencing at scale

More samples, more replicates.

Robust results, significant discoveries.

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Streamlined data analysis

Demultiplex and analyze BRB-seq data with our easy-to-use cloud-based pipeline.

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BRB-seq as a flexible solution

BRB-seq can be applied to libraries of any size, from a few samples to up-to 384 samples in one single tube.

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One-day lab workflow

From sample to sequencing-ready library in one day and proven compatibility with automated solutions.

The workflow


Discover more

User manual

How to use our positive control RNA


For use with (application)

3' mRNA sequencing

For use with (equipment)

Illumina NGS instruments

Validated species

Human, Mouse, Arabidopsis, Zebrafish, Drosophila

Species compatibility

All eukaryotic species

Available formats

24, 96, 384 reactions

Shipping conditions

Dry ice

Storage conditions



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